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Helicobacter pylori
Helicobacter pylori
規(guī)格:
貨期:
編號:B84182
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規(guī)格:
凍干粉
斜面
甘油
平板


產品名稱 Helicobacter pylori
商品貨號 B84182
中文名稱 幽門螺桿菌
模式菌株 模式菌株
其它保藏中心編號 BCRC15415,ATCC 43504; DSM 4867; JCM 7653; LMG 7539; NCTC 11637
特征特性 Nucleotide (GenBank) : AF053357 Helicobacter pylori glutamine synthetase (glnA) gene, complete cds. Nucleotide (GenBank) : AF053715 Helicobacter pylori GTP binding protein RgbA (rgbA) gene, partial cds. Nucleotide (GenBank) : AF010307 Helicobacter pylori AbcA (abcA), AbcB (abcB), AbcC (abcC) and AbcD (abcD) genes, complete cds. Nucleotide (GenBank) : AF125214 Helicobacter pylori cag pathogenicity island CagA (cagA) gene, partial cds; and urease-enhancing factor Lpp (lpp), urease-enhancing factor helB (helB), and HelA (helA) genes, complete cds. Nucleotide (GenBank) : AF125197 Helicobacter pylori 3-dehydroquinase type II AroQ (aroQ) gene, partial cds; hypothetical protein, small ribosomal subunit protein 15 RpsL15 (rpsL15), and flagellar biosynthesis/regulation protein FlbA (flbA) genes, complete cds; and hypoth Validation list no. 17. Int. J. Syst. Bacteriol. 35: 223-225, 1985. Marshall BJ, Goodwin CS. Revised nomenclature of Campylobacter pyloridis. Int. J. Syst. Bacteriol. 37: 68, 1987. Fox JG, et al. Campylobacter pylori subsp. mustelae subsp. nov. isolated from the gastric mucosa of ferrets (Mustela putorius furo), and an emended description of Campylobacter pylori. Int. J. Syst. Bacteriol. 38: 367-370, 1988. Goodwin CS, et al. Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. nov., respectively. Int. J. Syst. Bacteriol. 39: 397-405, 1989. Performance Standards for Antimicrobial Disk Susceptibility Tests; Approved Standard - 9th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M2-A9. Methods for Dilution Antimicrobial Susceptibility Tests for Bacteria that Grow Aerobically; Approved Standard - 7th Edition. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M7-A8. Yokota S, et al. Low antigenicity of the polysaccharide region of Helicobacter pylori lipopolysaccharides derived from tumors of patients with gastric cancer. Infect. Immun. 65: 3509-3512, 1997. PubMed: 9284113 Melchers K, et al. Cloning and membrane topology of a P type ATPase from Helicobacter pylori. J. Biol. Chem. 271: 446-457, 1996. PubMed: 8550601 Mendes EN, et al. Helicobacter trogontum sp. nov., isolated from the rat intestine. Int. J. Syst. Bacteriol. 46: 916-921, 1996. PubMed: 8863417 Bauerfeind P, et al. Allelic exchange mutagenesis of nixA in Helicobacter pylori results in reduced nickel transport and urease activity. Infect. Immun. 64: 2877-2880, 1996. PubMed: 8698529 Performance Standards for Antimicrobial Susceptibility Testing; Sixteenth Informational Supplement. Wayne, PA. Clinical and Laboratory Standards Institute; CLSI M100-S20, 2007 Medical microbiology--Culture media -- Part 4: Transport systems for specimens containing bacteria. Berlin, Germany:Deutsches Institut fur Normung;DIN DIN 58942-4: 2003, 2003
具體用途 科研,生產,主要用于介質測試,質量控制應變,敏感性試驗,原料藥產品的質量控制菌株,腸內研究
培養(yǎng)基 ATCC? Medium 18: Trypticase Soy Agar/Broth ATCC? Medium 260: Trypticase soy agar/broth with defibrinated sheep blood
培養(yǎng)溫度 37 ℃, 3-5%氧氣-10%二氧化碳
分離基物 人胃竇,澳大利亞
凍干物說明 ·凍干首次活化,干粉要全部用完,不能保留,用0.2-0.5m1的培養(yǎng)液或者無菌水溶解,接種在1-2個平板上,因凍干菌種處于休眠狀態(tài),請勿接種多個平板,以免因接種量不足而導致復蘇不成功;
保存說明 1、長期保存的儲存菌株以冷凍干燥保藏為主,特殊要求的菌種可用其它方法進行保存,保存期可長達幾年至幾十年。2、半儲存菌株以含20%甘油的肉湯或液體石臘封存的半固體瓊脂為主,在0℃以下保存,保存期為3-6個月。3、應用菌株以瓊脂斜面為主,在4℃保存,保存期為1-3個月。4、菌種的傳代不可超過6次。視菌種的特性及使用情況,一般儲存菌株每5年傳記代一次,每1-2年從儲存菌株轉接至半儲存菌株,半儲存菌株每3-6個月傳代一次,每1-3個月從半儲存菌株轉接接至應用菌株。
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